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human neuroblastoma cell lines chla255  (ATCC)


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    ATCC human neuroblastoma cell lines chla255
    Human Neuroblastoma Cell Lines Chla255, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1095 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+neuroblastoma+cell+lines+chla255/MEG-01/pmc12453546-26-1-17
    Average 97 stars, based on 1095 article reviews
    human neuroblastoma cell lines chla255 - by Bioz Stars, 2026-10
    97/100 stars

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    Cell Culture:

    Article Title: TIGIT affects CAR-NK cell effector function in the solid tumor microenvironment by modulating immune synapse strength
    Article Snippet: Recombinant human IL-4 (200–04-100UG), IL-6 (200–06-100UG), M-CSF (300–25-100UG), GM-CSF (300–03-100UG), and IL-15 (200–15-100UG) were purchased from Peprotech (Rocky Hill, NJ, USA). .. The human neuroblastoma cell lines CHLA255 (RRID:CVCL_AQ27, obtained 2021) and LA-N-1 (RRID:CVCL_1827, obtained 2017) were purchased from American Type Culture Collection (Manassas, VA, USA) and cultured in RPMI-1640 culture medium (Hyclone SH30096.01) supplemented with 2 mM L-glutamine (Gibco-BRL Cat# 35050061) and 10% FBS (Gibco-BRL Cat# A5670701). .. The human lung cancer cell line H1650 (RRID: CVCL_1483, obtained 2024) was purchased from American Type Culture Collection and cultured in complete-DMEM culture medium composed of DMEM medium (Hyclone Cat# SH30285.01) supplemented with 2 mM L-glutamine and 10% FBS.



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    human neuroblastoma cell lines chla255  (ATCC)
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    ATCC human neuroblastoma cell lines chla255
    Human Neuroblastoma Cell Lines Chla255, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+neuroblastoma+cell+lines+chla255/MEG-01/pmc12453546-26-1-17
    Average 97 stars, based on 1 article reviews
    human neuroblastoma cell lines chla255 - by Bioz Stars, 2026-10
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    chla255 human neuroblastoma cell line  (Pasteur Institute)
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    Pasteur Institute chla255 human neuroblastoma cell line
    Chla255 Human Neuroblastoma Cell Line, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human neuroblastoma cell line chla255  (DSMZ)
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    DSMZ human neuroblastoma cell line chla255
    The cytotoxicity assay of ADNKs vs PBNKs on the SK-N-SH and CHLA-255 <t>neuroblastoma</t> cell lines in the 1:1, 3:1, and 5:1 ratios (A) . Granzyme degranulation marker CD107a expression of natural killer cells (NKs) before (left side) and after (right side) NB cell stimulation was measured. It shows a significant correlation between degranulation amounts of ADNKs of the study groups ( p = 0.005). The profiles show CD107a surface staining of lysosomal proteins (sideward scatter), as indicated on CD3 − CD56 + (forward scatter) NK cells. The percentage of CD107a + cells (up right), CD56 + cells (down right), and CD3 + , CD4 + cells (left side) is indicated on each plot (B) . The data show the expanded NKs after stimulation with IL-21 can induce activation signaling to PBNKs and ADNKs. ELISA data have shown no changes in cytokine production in the absence of any stimulation on NK cells (C) but there is a decreased level of both interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) in ADNKs when compared to the normal PBNKs (D) . All data were statistically significant ( p < 0.05, each experiment was performed in triplicate). PBNKs, peripheral blood-derived NK cells; ADNKs, adipose tissue-derived NK cells.
    Human Neuroblastoma Cell Line Chla255, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+neuroblastoma+cell+lines+chla255/chla+255++dsmz+germany/pmc05681958-60-1-11
    Average 90 stars, based on 1 article reviews
    human neuroblastoma cell line chla255 - by Bioz Stars, 2026-10
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    The cytotoxicity assay of ADNKs vs PBNKs on the SK-N-SH and CHLA-255 neuroblastoma cell lines in the 1:1, 3:1, and 5:1 ratios (A) . Granzyme degranulation marker CD107a expression of natural killer cells (NKs) before (left side) and after (right side) NB cell stimulation was measured. It shows a significant correlation between degranulation amounts of ADNKs of the study groups ( p = 0.005). The profiles show CD107a surface staining of lysosomal proteins (sideward scatter), as indicated on CD3 − CD56 + (forward scatter) NK cells. The percentage of CD107a + cells (up right), CD56 + cells (down right), and CD3 + , CD4 + cells (left side) is indicated on each plot (B) . The data show the expanded NKs after stimulation with IL-21 can induce activation signaling to PBNKs and ADNKs. ELISA data have shown no changes in cytokine production in the absence of any stimulation on NK cells (C) but there is a decreased level of both interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) in ADNKs when compared to the normal PBNKs (D) . All data were statistically significant ( p < 0.05, each experiment was performed in triplicate). PBNKs, peripheral blood-derived NK cells; ADNKs, adipose tissue-derived NK cells.

    Journal: Frontiers in Immunology

    Article Title: Natural Killer Cells from the Subcutaneous Adipose Tissue Underexpress the NKp30 and NKp44 in Obese Persons and Are Less Active against Major Histocompatibility Complex Class I Non-Expressing Neoplastic Cells

    doi: 10.3389/fimmu.2017.01486

    Figure Lengend Snippet: The cytotoxicity assay of ADNKs vs PBNKs on the SK-N-SH and CHLA-255 neuroblastoma cell lines in the 1:1, 3:1, and 5:1 ratios (A) . Granzyme degranulation marker CD107a expression of natural killer cells (NKs) before (left side) and after (right side) NB cell stimulation was measured. It shows a significant correlation between degranulation amounts of ADNKs of the study groups ( p = 0.005). The profiles show CD107a surface staining of lysosomal proteins (sideward scatter), as indicated on CD3 − CD56 + (forward scatter) NK cells. The percentage of CD107a + cells (up right), CD56 + cells (down right), and CD3 + , CD4 + cells (left side) is indicated on each plot (B) . The data show the expanded NKs after stimulation with IL-21 can induce activation signaling to PBNKs and ADNKs. ELISA data have shown no changes in cytokine production in the absence of any stimulation on NK cells (C) but there is a decreased level of both interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) in ADNKs when compared to the normal PBNKs (D) . All data were statistically significant ( p < 0.05, each experiment was performed in triplicate). PBNKs, peripheral blood-derived NK cells; ADNKs, adipose tissue-derived NK cells.

    Article Snippet: The human neuroblastoma (NB) cell lines of SK-NSH (ATCC-HTB11TM) and CHLA255 (DSMZ-Germany), both were negative for MHC-I have been used in this study.

    Techniques: Cytotoxicity Assay, Marker, Expressing, Cell Stimulation, Staining, Activation Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay